Brine Shrimp Lethality of the Methanol Dichloromethane Extracts of Anonas (Annona reticulata) Leaves and Barks

Abstract

Leaves and barks of anonas (Anona reticulata) were collected from Vigan City, Ilocos Sur. The samples were chopped into fine pieces and then soaked in methanol. The extract was allowed to pass through a filter paper and the supernatant liquid was concentrated using a rotary evaporator for three hours at 40°C. Dichloremethane
was used to get the remaining concentrated extracts in the rotavapor and then evaporated in the fume hood.