The Antimicrobial Assay of Coumarins from Kakawate (Gliricidia sepium) Jacq. Kunth exWalph. Leaves against Staphylococcus aureus, Escherichia coli. and Trichophyton mentagrophytes

Abstract

To determine the active principle found in the leaves of kakawate (Gliricidia sepium) Jacq. Kunth. ex Walph., the kakawate leaves were soakedseparately in distilled water and petroleum ether. Pure compounds coumarins were isolated, characterized, and identified bymass spectroscopy (MS), ultra violet (UV) spectrophotometry, infra red (IR) spectrophotometry, proton (JH) and carbon 13 (13C) nuclear magnetic resonance (NMR) spectroscopy. Bioassay of the coumarins against microorganisms were tested against Escherichia coli, Staphylococcus aureus, and Trichophyton mentagrophytes. The coumarins manifested antimicrobial index of 14.5 against the fungus Trichophyton mentagrophytes.