The Antiangiogenic Activity of Alokon (Broussonetia luzonica) Flower Extract Using Chick Chorioallantoic Membrane (CAM) Assay

Abstract

Cancer remains a leading cause of mortality worldwide, with existing treatments often being costly and accompanied by adverse effects. This study investigated the antiangiogenic activity of Alokon (Broussonetia luzonica) flower extract as a potential natural alternative for inhibiting blood vessel formation critical to tumor growth. Using the Chick Chorioallantoic Membrane (CAM) assay, the study evaluated blood vessel inhibition across six treatment groups: distilled water (negative control), Quercetin (positive control), and four extract concentrations (25%, 50%, 75%, and 100%). Results demonstrated that Alokon extract exhibited a dose-dependent inhibition of angiogenesis, with the 100% concentration achieving comparable efficacy to Quercetin (27.87% and 29.60% inhibition, respectively). Statistical analyses, including ANOVA and Scheffé test, confirmed significant differences between treatment groups, highlighting the extract’s potential as a cost-effective antiangiogenic agent. These findings highlight the value of Alokon flower extract in developing accessible cancer therapies, warranting further exploration into its bioactive compounds and therapeutic applications.