Toxicity and Nutrient Testing of Local Roselle (Hibiscus sabdariffa L.) Fruit

Abstract

This study of the native roselle (Hibiscus sabdariffa) fruit was subjected to experimental and qualitative methods of research. It determined the nutrient content of roselle seeds and the lethal dose of the extract.Focus group discussion was used in gathering the needed data for the earlier traditional usage of the plant in the local community representing the households from the three (3) municipalities in Ilocos Sur, namely San Juan, Santa Catalina and Caoayan. Tests were conducted in the Industrial Testing Division Institute, Department of Science and Technology, Bicutan, Taguig City (ITDI, DOST). Results showed that the essential food nutrients of the seeds; per 100 g from the extract of the Roselle fruit are ash (5.36), protein (24.7), fat (18.2), total carbohydrates (48.6) and food energy, kcal/100g (457). In the toxicity testing (LD50), a total of 30 mice were used and given an increasing doses of 35, 40 and 45 grams per kilogram. Acute oral toxicity test showed no adverse or abnormal signs or death within the first 24 hours up to 14-day period of observation. Institutional Animal Care and Use Committee (IACUC) certificate was secured. Results of the focus group confirmed the value of the roselle plant to the local folks as a safe food to eat. To them, it is becoming a vanishing local crop.