Effect of Centrifugation Speed and Time on the Blood Chemistry Values of Healthy Individuals

Abstract

Blood chemistry testing requires centrifugation as one of the crucial pre-analytical phases. Usual differences in the speed and time of centrifugation influence specimens to varying extents among laboratories. This prompted the researchers to ascertain the effects of varying centrifugation speeds and times on healthy individuals' blood chemistry values and reduce the centrifugation time attested by the World Health Organization (WHO). Blood specimens were collected from four healthy adults, centrifuged, and then analyzed using the VITROS 5600 Automated Machine. The dry chemistry principle was applied. Three sets of speed and time were applied (1) 3,500 RPM for 15 minutes (WHO recommended), (2) 3,750 RPM for 10 minutes, and (3) 4,000 RPM for 5 minutes. The parameters include Fasting Blood Sugar (FBS), Total Cholesterol (Total CHOLE), Creatinine (Crea), Sodium (Na), and Potassium (K). The results of the serum chemistry values have no significant difference between different speeds and times against the standard set by WHO and were found to be acceptable, normal, and close in range with each other. Therefore, these can be substituted for the WHO-recommended speed and time. The researchers forward the following recommendations: (1) tests should be conducted to respondents with comorbidities and to younger and older individuals; (2) conduct the tests in triplicates and consider using other methods and more participants should be considered; and; (3) consider lesser time in centrifugation.